![]() Personnel and action plans would be executed in the first 100 days of a second term starting on Jan. But he has endorsed the work of several groups to prime an administration-in-waiting. Trump remains distracted by his obsession with contesting the 2020 election results. Most spoke on condition of anonymity to describe sensitive planning and avoid Trump’s ire.Īs Trump publicly flirts with a 2024 comeback campaign, this planning is quietly flourishing from Mar-a-Lago to Washington - with his blessing but without the knowledge of some people in his orbit. The reporting for this series draws on extensive interviews over a period of more than three months with more than two dozen people close to the former president, and others who have firsthand knowledge of the work underway to prepare for a potential second term. The heart of the plan is derived from an executive order known as “Schedule F,” developed and refined in secret over most of the second half of Trump’s term and launched 13 days before the 2020 election. Trump allies are working on plans that would potentially strip layers at the Justice Department - including the FBI, and reaching into national security, intelligence, the State Department and the Pentagon, sources close to the former president say.ĭuring his presidency, Trump often complained about what he called “the deep state.” The impact could go well beyond typical conservative targets such as the Environmental Protection Agency and the Internal Revenue Service. Former President Trump’s top allies are preparing to radically reshape the federal government if he is re-elected, purging potentially thousands of civil servants and filling career posts with loyalists to him and his " America First” ideology, people involved in the discussions tell Axios.
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These present results suggest that this new analytical tool can be used as a supporting method for quantification of intracellular protein. Furthermore, Rab5 puncta of PARK8 patient iPSC-derived neurons exhibited distinct localization pattern relative to isogenic iPSC-derived neurons. On the other hands, at 14 days in vitro, the numbers of Rab5 puncta in PARK8 patient iPSC-derived neurons were lower than those of isogenic iPSC-derived neurons, but not Rab7 puncta. Interestingly, in long-term culture, we found that the numbers of Rab7 puncta in a single PARK8 patient iPSC-derived neurons were lower than that of control iPSC-derived neurons. These were able to successfully detect Rab5 puncta and Rab7 puncta in PARK8 patient iPSC-derived neurons. ![]() Finally, we developed new pipelines for analysis of disease phenotype using iPSCs from a patient with familial Parkinson's disease (PD), harboring the I2020T LRRK2 mutation (PARK8). In addition, when applied to quantitative measurement of phagocytosis, our pipelines clearly detected monocytic cell lines that had engulfed bioparticles. Using CellProfiler and ImageJ in combination, we confirmed that our pipelines were applicable both quantitatively and objectively to analysis of membrane trafficking of proteins such as Rab proteins and transferrin. We then constructed pipelines for measuring the distance from the center of the nucleus to allow investigation of the intracellular localization of Rab7 or transferrin. ![]() This revealed that scattered puncta of Rab7 and transferrin in neuroblastoma lines were clearly detectable by created analytical pipelines in CellProfiler. In the present study, to examine whether intracellular proteins can be discriminated using a combination of CellProfiler and ImageJ, we analyzed neuroblastoma and monocytic cell lines, and disease-specific induced pluripotent stem cell (iPSC)-derived neurons. If you are an interested developer you can register for free to get access to the CZI documentation.Analytical pipeline, which is used for various analysis application, of CellProfiler, an open-source software for cell imaging analysis, is very important. Instead, ZEISS supports open software projects and communities with developer-level access, documentation and SDKs for selected microscopy products. Please understand that ZEISS Microscopy cannot provide any kind of user-level assistance for these applications. CZI image and meta-data import is supported through the Bio-Formats plug-in of the Open Microscopy Environment. Precompiled installation files of these open source imaging programs are available for Mac OS X and a number of Linux distributions, as well as a multitude of plug-ins for analysis and processing. If you wish to view and process data that has been created in this format on non-Windows plattforms we recommend to visit the project homepages of ImageJ and the ImageJ-based Fiji software package. ![]() Images created with ZEN software and ZEISS microscopy systems are saved in the fully documented file format CZI. To support you on other operating systems such as Mac OS X or Linux, ZEISS offers documentation for interoperability and in addition established cooperations with partner projects and companies. ZEN software and microscopy imaging workstations by ZEISS are designed to run on 64bit Microsoft Windows platforms. ZEISS Nanoscale and Correlative Analysis. ![]() Equipped with a collection of three imposing prpducts issued by Corel which are Corel Photo-Paint, DRAW and Connect.Can design as well as create staggering logos, ads and websites.An impressive and professional design suite that has been equipped with the options to help you in creating some outstanding projects.You can also download CorelDRAW Graphics Suite X5 2010.įeatures of CorelDRAW Graphics Suite 2019 for Macbookīelow are some main features which you’ll experience after CorelDRAW Graphics Suite 2019 for Mac free download. On a conclusive note we can say that CorelDRAW Graphics Suite 2019 for Mac OS X is an impressive and professional design suite that has been equipped with the options to help you in creating some outstanding projects. ![]() Corel Photo-Paint lets you perform pixel editing on the bitmap images and Corel Connect lets you search as well as manage photos, clip arts and fonts. You can adjust the images and can add various special effects like blur, color transform, distort and sharpen. It has got a classic, menu-shaped user interface. CorelDRAW lets you to explore your creativity and to express the artist in an intuitive and comfortable environment. You can also download CorelDRAW Graphics Suite X3.ĬorelDRAW Graphics Suite 2019 for Mac OS X has been equipped with a collection of three imposing prpducts issued by Corel which are Corel Photo-Paint, DRAW and Connect. This application has been developed for the professional designers and artists. With this application you can design as well as create staggering logos, ads and websites. Latest Version setup of CorelDRAW Graphics Suite 2019 Premium Pro DMG v21.0.0.593.īrief Overview of CorelDRAW Graphics Suite 2019 for Mac OS XĬorelDRAW Graphics Suite 2019 for Mac OS X is an impressive and professional design suite that has been equipped with the options to help you in creating some outstanding projects. Download CorelDRAW Graphics Suite 2019 for Mac Free. She’s a nice girl with a strong spirit but very vulnerable as well. ![]() ![]() ![]() She’s cute, though not particularly beautiful, and pretty much average in every other way. Mei is your standard "everyman" kind of girl typical in shoujo romances. A romance is by nature very character driven and if we don’t get compelling leads it’s pretty difficult to care if they end up falling in love with each other. Much in the same way as the story is underwhelming, so are the series main characters. But when you compare it with its peers within the genre and even shows that were airing during the same time as this, it comes across as distinctly average and ordinary. The show is called Say I love you, but it should probably more aptly called Say why I love you.Įven though I have some pretty serious complaints about the way the shows romance and plot unfolds, I can’t say I didn’t enjoy watching this series. While it can often be frustrating when it is drug out with silly misunderstandings and character cockblocks at least by then we have a clear understanding why they both love each other. The typical format is 99% courting with the eventual payoff at the end when our little lovebirds finally realize their mutual affections. For the most part our lead characters are in a relationship together from the beginning to the end of the series. Now here is where Suki na yo is a bit different from its other romance cousins. However this is sadly mostly wasted over the course of the story. The girl Mei, and the boy Yamato, both have some interesting personality issues on the surface and despite the silliness of their meeting there was some enormous amount of potential in their relationship. The beginnings of true and lasting love if I ever saw it. Suki na yo is no different, with the unlikely story of the hot and most popular guy in school falling for a shy, friendless loner who kicked him down the stairs because she mistakenly thought he flipped her skirt. It’s hard for me to be too hard on the romance anime plot premise, I mean they have pretty much already all been done before and But as the series winds down I found myself lamenting what could have been. It really is a shame, because Suki na yo had a tremendous amount of potential and gave us some things we don’t often get to see in the romance genre. but I just can’t because you’re a flat two dimensional romance that just didn’t live up to my expectations. ![]() Oh how I wanted to say, I love you to Sukitte Ii na yo. Throughout misunderstandings of their new relationship, each other, and the attentions of other girls, Mei and Yamato slowly grow closer and learn the true meaning of those three little words: "I love you." Mei, however, finds it very hard to adapt to this new lifestyle, especially in expressing her true feelings towards Yamato. On the heels of her discovery that their feelings are mutual, they start dating and she gains not only a boyfriend, but friends as well. But when a dangerous situation ends with Yamato kissing Mei to save her from the unwanted attention of a stalker, Mei begins to develop feelings for him. Yamato finds her intriguing and insists on being her friend, even though Mei wants nothing to do with him. However, Mei's life begins to change drastically when a misunderstanding in high school causes her to encounter popular student Yamato Kurosawa. Since then, she has stayed away from people in order to avoid ever being hurt again. Friends will only let you down-that is the sad truth Mei Tachibana lives with, ever since she was wrongfully blamed for the death of a class pet by her so-called friends in grade school. ![]() It will always be an evolving community cookbook this year as I will always be adding in extra pages if anyone else submits recipes or I add a few more. Then tomorrow I’ll need to write up a few pages, add in some conversion charts (as we have recipes from different continents) and finally proof read it all then upload it to my website. It is more time consuming than I imagined but I’ve made great headway and I hope to have every single submitted recipe in and laid out nicely by the end of the day. I’m still busy adding in your recipes and laying the pages out. The first one on my list for 2022 is to finish the first version of the ‘Pandemic Pantry’ cookbook. Love this time of year as it can feel like a good time to set positive goals. HAPPY NEW YEAR!!!! Wishing everyone a very happy and of course healthy 2022. I'll put in another few hours today, I'm back to work tomorrow and will just do my best and realistically aim to get this completed by the end of next weekend. In the end we will have around 70 in total. I have another 20+ to do plus write up and charts. I'm putting in quite a few hours each day but it takes me around 20 minutes to lay out a recipe. PS: I’ll be doing some of the Pandemic Pantry recipes on my YouTube channel now and again so please subscribe HERE! Thank you.ģrd JANUARY UPDATE: It really is taking me longer than I thought. ![]() Sending much love and safety to all during these times of uncertainty, whether its medically, economically or with the recent war in Ukraine and the worry of escalation into neighbouring countries, I sincerely wish you all the very best. I really want to say THANK YOU to those who sent through their recipes, at last count I was up to 180 something so there will be lots to add over the coming months. ![]() I sometimes feel like I am trying to climb a greasy pole to complete things as I am time challenged and the last few years, well, what can I say, we’ve all been coping with many things in different ways. The Pandemic Pantry cookbook will ALWAYS be free and if anyone would like to send any frugal recipes through please do so to sincerely APOLOGISE for the time it has taken me. If you do not see the recipe you submitted please know that it will be added, just download your new version every month. It will be much easier for me to add in several new recipes every month and update the book with valuable resources and links to keep it current. I’ve decided that on the 1st day of each calendar month there will be a NEW version of the cookbook FREE to download. FINALLY the “Pandemic Pantry Community Cookbook” is ready to DOWNLOAD.īefore you dive in I’d first like to say that this first version contains 38 of the nearly 200 recipes already submitted during the pandemic BUT my life is hectic, my home circumstances right now are challenging and if I didn’t do it this way it would NEVER get finished. |
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